Journal: The Journal of Biological Chemistry
Article Title: Myeloid-derived growth factor is a resident endoplasmic reticulum protein
doi: 10.1074/jbc.AC118.002052
Figure Lengend Snippet: Analysis of MYDGF colocalization with organelle markers Colocalization was quantified as the area of overlapping signal from goat anti-MYDGF (Alexa Fluor 488–conjugated secondary antibody) and rabbit anti-MYDGF or rabbit antibodies to organelle markers (Alexa Fluor 647–conjugated secondary antibody) divided by the signal area from one antibody alone. Values are reported as averages from 9–16 0.3-μm slices from fields of 21–41 eosinophils that were overlaid to create the maximal intensity projections from which the cells shown in were chosen. Standard deviations represent variation among the slices.
Article Snippet: Primary antibodies were used at 2 μg/ml for goat anti-MYDGF, 0.2 μg/ml for rabbit anti-MYDGF, and 1:1000 dilution for rabbit anti-P4HB (3501, Cell Signaling Technology).
Techniques: